Team introduction
The green manufacturing team of biological bacterium agent and bio based chemicals of Dalian University of Technology is headed by Professor Xue Chuang, a young Changjiang scholar from the Ministry of Education, whose team has longbeen committed to the applied basic research in synthetic biology. The team members have undertaken 10 national key R&D projects (topics), NSFC programs, 12 provincial and ministerial projects (topics), published more than 100 papers in international authoritative journals such as Nano Lett, Nature Commun and others, authorized more than 20 Chinese invention patents. As for reward, the team won the first prize of China Renewable Energy Society for Science and Technology Progress, the gold prize of the 48th Geneva International Invention Exhibition, the first prize of Liaoning Province for Science and Technology Progress, the second prize of Liaoning Province for Natural Science, and the first prize of China Federation of Commerce for Science and Technology. The transformation of research achievements serves Sinopec, SDIC Bioenergy, Shandong Chambo Chemical, Shandong Chambo Boxing Tianzhu, Jiangsu Huaying Shunchang, Dalian Huadian, Dalian Xinyulong and other enterprises.
The research team used synthetic biological gene editing, high-throughput screening, biological fermentation and other technologies to select production strains, and produced bio based chemicals such as cannabinol, butanol, butanedione and fatty acid through efficient microbial catalytic transformation for biomedical and functional food industries; The selected probiotics are used to prepare biological bacterium agent to realize the recycling and energy utilization of waste biomass, which can be used to produce bio fertilizer, bio ethanol, bio natural gas and other modern agriculture, energy and environmental protection industries.
Team promotion video
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Team leader
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Professor Xue Chuang: doctoral supervisor, young scholar of the "Changjiang Scholars Program of China" of the Ministry of Education, expert in the compilation of key special guidelines for national key R&D programs, selected as one of the first batch of academic leaders in Liaoning Province, one of the first batch of young top talents in the "rejuvenating Liaoning talents program" of Liaoning Province, Liaoning Youth Science and Technology Award (Top Ten Talents), Liaoning Province's outstanding scientific and technological workers, and Liaoning Province's innovative talents support program for colleges and universities. In recent five years, he has presided over more than 10 national key R&D projects, sub projects and NSFC programs, published more than 50 academic papers, cited more than 100 times, authorized 10 Chinese invention patents and 2 PCT international patents, converted 3 patent licenses. Meanwhile, he won the first prize of Science and Technology Progress of China Renewable Energy Society, the first prize of Science and Technology Progress of Liaoning Province, the gold prize of the 48th Geneva International Invention Exhibition, the first prize of Liaoning Natural Science, the first prize of Science and Technology Progress of China Federation of Commerce and a number of international academic awards. |
Team members
Team research achievements and application fields
1. Biological bacterium agent and biological organic fertilizer
Bacillus, Trichoderma, Streptomyces, and other functional microorganisms and probiotics were selected, and multi-bacteria mixed high-density fermentation and process control technology was established to improve the level of growth promotion and antibacterial active product synthesis for developing high-titer composite biological bacterium agent with synergistic growth promotion, disease prevention and disease resistance functions. Using biological bacterium agent, biological organic fertilizer with humic acid chelated boron, magnesium, zinc and other medium and trace elements was invented and prepared by aerobic fermentation of biogas residues, stalks, dung and other raw materials, which reduced the incidence of common crops diseases such as little leaf disease and nematode by more than 70%. Relevant technologies have reached the international first-class level, and have achieved large-scale production and application in Shandong Jingbo Boxing Tianzhu, Dalian Huadian Biology and Dalian Xinyulong. It has achieved good social and economic benefits.
2. Biofuels and bio natural gas
A multi gene editing method for multi-plasmid co transformation was created. Based on this method, ultra-high concentration fermentation technology and fermentation wastewater recycling technology were developed, as well as a new methane fermentation increasing technology for stalks raw material pretreatment and CO2 recycling by selecting new strains with enhanced product synthesis ability, such as Yeast, Clostridium and Trichoderma. Relevant technologies were applied to SDIC bioenergy production with an annual output of 300000 tons of bioethanol liquid fuel, and Jiangsu Huaying Shunchang million cubic meters biogas/biological natural gas project and 10000 tons of biological bacterium agent production.
3. Microbial fermentation to produce cellulase
MGD(a mixture of glucose and sophorose), a cheap and efficient inducer, was prepared by transglycoside reaction. Based on the characteristics of cell growth and metabolism, the fed-batch of inducer was controlled to induce cellulase production efficiently; Genetic modification of Trichoderma reesei was carried out to improve the hydrolysis efficiency of lignocellulose and the total sugar yield of crude and compound enzyme solutions. Relevant technologies were applied in Sinopec Dalian Petrochemical Research Institute. The cellulase produced by Trichoderma reesei genetic engineering strain is superior to commercial enzymes in some enzyme production indicators, which contributies to breaking the foreign monopoly of cellulase and serving the development of national energy and environmental protection industries.
4. Synthetic biological natural products and pharmaceutical products
Using biological chassis cells such as Saccharomyces cerevisiae and Escherichia coli, the full synthesis of natural products and medical products (cannabinol, CBD) was realized starting from monosaccharides. The metabolic pathway and fermentation method were optimized to obtain high-yield strains. The gene of synthetic CBD from plants and heterologous microorganisms is introduced into Saccharomyces cerevisiae by synthetic biological technology, and a thousand square meters fermentation workshop can be used to replace ten thousand mu of good farmland to produce cannabinol, which is a pioneering work in China and has cross era significance.
5. Use CO2 to synthesize bioenergy and bio based chemicals
Clostridium carboxidivorans with efficient utilization of CO2 was selected. Theanaerobic microorganisms, fixing CO2 under non photosynthesis, was modified to improve the CO2 conversion efficiency by metabolic engineering. The coupling integration technology of microbial electrosynthesis and CO2 recycling was developed to achieve efficient biocatalysis conversion of a variety of biofuels and bio based chemicals.
6. Efficient separation and purification of alcohols and natural gas products
The organic composite membrane with carbon nanotubes opening and array structure was designed and fabricated, which solved the problems of carbon nanotubes blending growth, etching opening, impurity removal in the tube, multi-layer coating, etc., and realized the cross scale precise control of the membrane pore. The results of density functional theory(DFT) calculations and experiments revealed the selective permeation mechanism of methane, CO2, alcohols and other molecules. The membrane broke through the bottleneck of the traditional membrane flux and selectivity, and established a high concentration fermentation coupling integration technology.
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